TRI Reagent® Sigma

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA. - The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Upstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA. - The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

RNA was extracted using TRI-Reagent (Sigma, St.Louis MO, USA), phenol/chloroform extraction and were purified with RNeasy spin column kit (Qiagen, Venlo, The Netherlands). cDNA synthesis was performed using the ‘Thermo Script RT-PCR System’ and random primer hexameres (Invitrogen, San Diego CA, USA).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized U373 using TRI Reagent® Sigma from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TRI Reagent® Sigma below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - immortalized U373 using TRI Reagent® Sigma from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.