QIAamp DNA Blood Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

Genome sequencing and comparison analysis
Genome DNA of each strain was extracted using a QIAamp DNA Blood Mini Kit (Qiagen). The 250-bp paired-end read sequencing of seven sets of MRSA-originating strains (MR/VR1, 2, 3, 4, 5, 7, 8) and a set of RN4220-originating strains (RN4220 and VR-RN) was performed using the MiSeq sequencing platform (Illumina) according to a previous report12. Comparison of genome sequences using CLC Genomics Workbench 7.5 (CLC bio) was performed as follows. First, each genome sequence was compared with the reference genome. The paired genome sequences of each MRSA strain was subjected to the multi-locus sequence typing analysis using a program provided by Center of Genomic Epidemiology (http://www.genomicepidemiology.org). Five strains (MR1, 3, 5, 7, 8) were categorized as ST5, and two strains (MR2, 4) were assigned as ST8. Because N315 (accession No. NC_002745.2) and USA300_FPR3757 (accession No. NC_007793.1) are representative strains of ST5 and ST8, respectively, whose genomes have been characterized, they were used for reference genomes. Second, the difference within coding regions between each set of strains was extracted.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram positive Staphylococcus aureus using QIAamp DNA Blood Mini Kit from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Blood Mini Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA isolation / purification Bacteria - Gram positive Staphylococcus aureus using QIAamp DNA Blood Mini Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.