RNAzol® RT

RNA isolation / purification Cells - immortalized U87

Experiment
RNA isolation / purification Cells - immortalized U87
Product
RNAzol® RT from Molecular Research Center, Inc.
Manufacturer
Molecular Research Center, Inc.

Protocol tips

Upstream tips
- To reduce RNA degradation do not wash cells before the addition of RNAzol® RT.

- To reduce DNA contamination, use sufficient amount of RNAzol® RT based on the area of the culture dish and not on cell number.
Protocol tips
To reduce DNA contamination extend the incubation time to 15 minutes before sedimentation.

Publication protocol

RNAzol RT (Molecular Research Center,
USA) was used to extract total RNA from glioma
tissue, adjacent normal tissue, glioma cell lines,
HBMECs and exosomal sources24. qRT-PCR for
human-specific repeat sequences was performed

Full paper   Login or join for free to view the full paper.

Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

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Manufacturer protocol

Download the product protocol from Molecular Research Center, Inc. for RNAzol® RT below.

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Videos

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