RNAzol® RT

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- To reduce RNA degradation do not wash cells before the addition of RNAzol® RT. - To reduce DNA contamination, use sufficient amount of RNAzol® RT based on the area of the culture dish and not on cell number.	To reduce DNA contamination extend the incubation time to 15 minutes before sedimentation.

Upstream tips
- To reduce RNA degradation do not wash cells before the addition of RNAzol® RT. - To reduce DNA contamination, use sufficient amount of RNAzol® RT based on the area of the culture dish and not on cell number.
Protocol tips
To reduce DNA contamination extend the incubation time to 15 minutes before sedimentation.

Publication protocol

RNAzol RT (Molecular Research Center,
USA) was used to extract total RNA from glioma
tissue, adjacent normal tissue, glioma cell lines,
HBMECs and exosomal sources24. qRT-PCR for
human-specific repeat sequences was performed

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized U87 using RNAzol® RT from Molecular Research Center, Inc..

Manufacturer protocol

Download the product protocol from Molecular Research Center, Inc. for RNAzol® RT below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - immortalized U87 using RNAzol® RT from Molecular Research Center, Inc.. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.