RNeasy Plus Mini Kit

RNA isolation / purification Cells - immortalized U87

Experiment
RNA isolation / purification Cells - immortalized U87
Product
RNeasy Plus Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

otal RNA was isolated from the cells or tissues using an RNeasy Plus Mini kit (Qiagen, Valencia, CA). One microgram total RNA and random primers were used for cDNA synthesis using the AffinityScript qPCR cDNA synthesis kit (Agilent Technologies, Santa Clara, CA).

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized U87 using RNeasy Plus Mini Kit from Qiagen.

Paper title
Glioma Big Potassium Channel Expression in Human Cancers and Possible T Cell Epitopes for Their Immunotherapy
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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