RNeasy Plus Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

otal RNA was isolated from the cells or tissues using an RNeasy Plus Mini kit (Qiagen, Valencia, CA). One microgram total RNA and random primers were used for cDNA synthesis using the AffinityScript qPCR cDNA synthesis kit (Agilent Technologies, Santa Clara, CA).

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Mini Kit below.

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