TriPure Isolation Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low contamination rate carefully remove the upper aqueous phase . - Adding too little reagent can lead to contamination with DNA.

Protocol tips
- For low contamination rate carefully remove the upper aqueous phase . - Adding too little reagent can lead to contamination with DNA.

Publication protocol

Isolation of total RNA, reverse transcription into cDNA and Real-time PCR reactions were performed as published before [9], using CFX Real-time PCR System (Bio-Rad Laboratories Inc., CA)

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TriPure Isolation Reagent below.

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