DNeasy Blood & Tissue Kit

DNA isolation / purification Bacteria - Gram negative Legionella

Experiment

DNA isolation / purification Bacteria - Gram negative Legionella

Product

DNeasy Blood & Tissue Kit from Qiagen

Manufacturer

Qiagen

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA extraction and PCR
For extraction of DNA from the filter sandwiches, a modified DNeasy protocol (Qiagen, Hilden, Germany) was used as described in detail by Henne et al. (2013). Quantification of DNA was carried out using Picogreen (dsDNA quantification, Molecular Probes; Invitrogen) according to the manufacturer.

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Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

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Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative Legionella using DNeasy Blood & Tissue Kit from Qiagen.

Paper title

species diversity and dynamics from surface reservoir to tap water: from cold adaptation to thermophily

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Manufacturer protocol

Download the product protocol from Qiagen for DNeasy Blood & Tissue Kit below.

Download manufacturer protocol

Videos

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