DNeasy Blood & Tissue Kit

DNA isolation / purification Bacteria - Gram negative Legionella

Experiment
DNA isolation / purification Bacteria - Gram negative Legionella
Product
DNeasy Blood & Tissue Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA extraction and PCR
For extraction of DNA from the filter sandwiches, a modified DNeasy protocol (Qiagen, Hilden, Germany) was used as described in detail by Henne et al. (2013). Quantification of DNA was carried out using Picogreen (dsDNA quantification, Molecular Probes; Invitrogen) according to the manufacturer.

Full paper   Login or join for free to view the full paper.

Reviews

DNeasy Blood & Tissue Kit from Qiagen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative Legionella using DNeasy Blood & Tissue Kit from Qiagen.

Paper title
species diversity and dynamics from surface reservoir to tap water: from cold adaptation to thermophily
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Qiagen for DNeasy Blood & Tissue Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA isolation / purification Bacteria - Gram negative Legionella using DNeasy Blood & Tissue Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms