RNeasy Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Total RNA from C6/36 cells at rest was isolated with RNAeasy (Qiagen). Total RNA from cells at rest was enriched for messenger RNA with oligo dT dynabeads (Invitrogen, Carlsbad, CA, USA). Total RNA from mock-infected and Zika-infected samples was subjected to SISPA multiplex library construction [53] and sequenced by Illumina NextSeq 1 × 150-bp sequencing.

Protocol tips
Total RNA from C6/36 cells at rest was isolated with RNAeasy (Qiagen). Total RNA from cells at rest was enriched for messenger RNA with oligo dT dynabeads (Invitrogen, Carlsbad, CA, USA). Total RNA from mock-infected and Zika-infected samples was subjected to SISPA multiplex library construction [53] and sequenced by Illumina NextSeq 1 × 150-bp sequencing.

Publication protocol

Total RNA from C6/36 cells at rest was isolated with RNAeasy (Qiagen). Total RNA from cells at rest was enriched for messenger RNA with oligo dT dynabeads (Invitrogen, Carlsbad, CA, USA). Total RNA from mock-infected and Zika-infected samples was subjected to SISPA multiplex library construction [53] and sequenced by Illumina NextSeq 1 × 150-bp sequencing.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized C6/36 using RNeasy Mini Kit from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - immortalized C6/36 using RNeasy Mini Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.