RNeasy Mini Kit

RNA isolation / purification Cells - immortalized C6/36

Experiment
RNA isolation / purification Cells - immortalized C6/36
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
Total RNA from C6/36 cells at rest was isolated with RNAeasy (Qiagen). Total RNA from cells at rest was enriched for messenger RNA with oligo dT dynabeads (Invitrogen, Carlsbad, CA, USA). Total RNA from mock-infected and Zika-infected samples was subjected to SISPA multiplex library construction [53] and sequenced by Illumina NextSeq 1 × 150-bp sequencing.

Publication protocol

Total RNA from C6/36 cells at rest was isolated with RNAeasy (Qiagen). Total RNA from cells at rest was enriched for messenger RNA with oligo dT dynabeads (Invitrogen, Carlsbad, CA, USA). Total RNA from mock-infected and Zika-infected samples was subjected to SISPA multiplex library construction [53] and sequenced by Illumina NextSeq 1 × 150-bp sequencing.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized C6/36 using RNeasy Mini Kit from Qiagen.

Paper title
Analysis of the C6/36 genome provides insight into cell line utility for viral propagation
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

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