TRIzol Reagent

Protocol tips

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	Total RNA was isolated by using TRI-Reagent (Molecular Research Centre) at different time points after infection, and enriched for small RNAs by using PureLink miRNA isolation kit (Invitrogen).

Protocol tips
Total RNA was isolated by using TRI-Reagent (Molecular Research Centre) at different time points after infection, and enriched for small RNAs by using PureLink miRNA isolation kit (Invitrogen).

Publication protocol

Total RNA was isolated by using TRI-Reagent (Molecular Research Centre) at different time points after infection, and enriched for small RNAs by using PureLink miRNA isolation kit (Invitrogen).

Cytoplasmic and nuclear fractions were isolated by using PARIS kit (Ambion) according to the manufacturer’s instructions, and TRI-Reagent was used for RNA extraction.

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Manufacturer protocol

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