RNeasy Protect Cell Mini Kit

RNA isolation / purification Cells - immortalized C6/36

Experiment
RNA isolation / purification Cells - immortalized C6/36
Product
RNeasy Protect Cell Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
Detached cells were transferred into a sterile 2 ml tube and were stored immediately at −80°C until total RNA extraction. Cells were homogenized in 350 µl RLT buffer in QIAshredder spin columns (Qiagen, Hilden, Germany) prior to total RNA extraction with Qiagen RNeasy Protect cell mini kit (Qiagen) according to manufacturer's instructions.

Publication protocol

Upon infection, C6/36 cells were harvested at 0 min, 15 mins, 30 mins and 120 mins post infection (pi). At 0 min pi, cells were harvested immediately upon virus inoculation. At each time point, C6/36 cells were washed with 2 ml of the pre-warmed (28°C) maintenance medium. After decanting the maintenance medium, 1 ml of Qiagen Cell Protect solution was added to each flask. Detached cells were transferred into a sterile 2 ml tube and were stored immediately at −80°C until total RNA extraction. Cells were homogenized in 350 µl RLT buffer in QIAshredder spin columns (Qiagen, Hilden, Germany) prior to total RNA extraction with Qiagen RNeasy Protect cell mini kit (Qiagen) according to manufacturer's instructions.

Full paper   Login or join for free to view the full paper.

Reviews

RNeasy Protect Cell Mini Kit from Qiagen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized C6/36 using RNeasy Protect Cell Mini Kit from Qiagen.

Paper title
Mosquito Cellular Factors and Functions in Mediating the Infectious entry of Chikungunya Virus
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Protect Cell Mini Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - immortalized C6/36 using RNeasy Protect Cell Mini Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms