RNeasy Plus Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Concentrated (1,000×) Sf9 cell supernatant was prepared by pooling medium from cells at confluence, filtering (pore size, 0.45 μm; Corning, Corning, NY), and ultracentrifugation at 125,000 × g for 20 h at 4°C. RNAs were extracted from 40 μl of 1,000×-concentrated Sf9 cell supernatant or from 116 μl of 1×-concentrated supernatant using a QIAamp viral RNA minikit (Qiagen, Germantown, MD) after DNase digestion (Promega, Madison, WI) by incubation at 37°C for 60 min. Total cell RNAs were prepared from a pellet of cultured cells or from a vial of frozen cells using an RNeasy Plus minikit (Qiagen). cDNA syntheses from supernatant RNAs or cell RNAs were done using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA).

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Mini Kit below.

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