RNeasy Plus Mini Kit

RNA isolation / purification Cells - immortalized Sf21

Experiment
RNA isolation / purification Cells - immortalized Sf21
Product
RNeasy Plus Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Concentrated (1,000×) Sf9 cell supernatant was prepared by pooling medium from cells at confluence, filtering (pore size, 0.45 μm; Corning, Corning, NY), and ultracentrifugation at 125,000 × g for 20 h at 4°C. RNAs were extracted from 40 μl of 1,000×-concentrated Sf9 cell supernatant or from 116 μl of 1×-concentrated supernatant using a QIAamp viral RNA minikit (Qiagen, Germantown, MD) after DNase digestion (Promega, Madison, WI) by incubation at 37°C for 60 min. Total cell RNAs were prepared from a pellet of cultured cells or from a vial of frozen cells using an RNeasy Plus minikit (Qiagen). cDNA syntheses from supernatant RNAs or cell RNAs were done using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA).

Full paper   Login or join for free to view the full paper.

Reviews

RNeasy Plus Mini Kit from Qiagen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized Sf21 using RNeasy Plus Mini Kit from Qiagen.

Paper title
Identification of a Novel Rhabdovirus in Cell Lines
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Mini Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - immortalized Sf21 using RNeasy Plus Mini Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms