TRIzol Reagent

RNA isolation / purification Cells - immortalized Sf9

Experiment
RNA isolation / purification Cells - immortalized Sf9
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Approximately 5 × 106 Sf9 cells were collected, and the total RNA was isolated following the manufacturer’s instructions using TRIzol reagent (Invitrogen, USA). RNA degradation and contamination were assessed using 1% agarose gels. RNA purity was detected using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). An RNA Nano 6000 Assay Kit was used to assess the RNA integrity using the Agilent Bioanalyzer 2100 system (Agilent Technologies, USA). The total RNA of three biological replicates was mixed together, and 3 μg of mixed RNA was used for the RNA sample preparation. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s instructions.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized Sf9 using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Transcriptome analysis of Sf9 cells reveals putative apoptosis-related genes and a preliminary apoptosis mechanism induced by azadirachtin
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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