miRNeasy Mini kit

RNA isolation / purification Cells - immortalized Sf9

Experiment
RNA isolation / purification Cells - immortalized Sf9
Product
miRNeasy Mini kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To capture more amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step

Publication protocol

Total cellular RNA was extracted from Sf9L5814 cells (5 x 106 cells) in a late growth phase culture (2.0–3.0 x 106 cells/ml, 98% cell viability) by using the miRNeasy mini kit (Qiagen, Valencia, CA). One-step RT-PCR was performed on total cellular RNA using SuperScript One-Step RT-PCR with Platinum® Taq (Thermo Fisher Scientific, Waltham, MA)

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized Sf9 using miRNeasy Mini kit from Qiagen.

Paper title
Complete study demonstrating the absence of rhabdovirus in a distinct Sf9 cell line
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Manufacturer protocol

Download the product protocol from Qiagen for miRNeasy Mini kit below.

Download PDF Download manufacturer protocol

Videos

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