Aquadien™ DNA Extraction Kit

DNA isolation / purification Bacteria - Gram negative Legionella

Experiment
DNA isolation / purification Bacteria - Gram negative Legionella
Product
Aquadien™ DNA Extraction Kit from Bio-Rad Laboratories
Manufacturer
Bio-Rad Laboratories

Protocol tips

Downstream tips
Wait for 48 hrs after biocide treatment before sampling 1 L of water that will be submitted to
real-time PCR analysis.

Publication protocol

DNA was extracted for qPCR analysis from 450 mL of the sampled water using the BIO-RAD Aquadien™ Kit following manufacturer’s instructions giving a final volume of 100 µL of DNA extract (Bio-Rad Laboratories, Inc., Sydney, NSW, Australia). Triplicate qPCR was then performed for the enumerations of Legionella spp., L. pneumophila and MAC.

Full paper   Login or join for free to view the full paper.

Reviews

Aquadien™ DNA Extraction Kit from Bio-Rad Laboratories has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative Legionella using Aquadien™ DNA Extraction Kit from Bio-Rad Laboratories.

Paper title
Detection of , and Mycobacterium Avium Complex (MAC) along Potable Water Distribution Pipelines
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Bio-Rad Laboratories for Aquadien™ DNA Extraction Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA isolation / purification Bacteria - Gram negative Legionella using Aquadien™ DNA Extraction Kit from Bio-Rad Laboratories. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms