Aquadien™ DNA Extraction Kit

DNA isolation / purification Bacteria - Gram negative Legionella

Experiment

DNA isolation / purification Bacteria - Gram negative Legionella

Product

Aquadien™ DNA Extraction Kit from Bio-Rad Laboratories

Manufacturer

Bio-Rad Laboratories

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		Wait for 48 hrs after biocide treatment before sampling 1 L of water that will be submitted to real-time PCR analysis.

Downstream tips
Wait for 48 hrs after biocide treatment before sampling 1 L of water that will be submitted to real-time PCR analysis.

Publication protocol

DNA was extracted for qPCR analysis from 450 mL of the sampled water using the BIO-RAD Aquadien™ Kit following manufacturer’s instructions giving a final volume of 100 µL of DNA extract (Bio-Rad Laboratories, Inc., Sydney, NSW, Australia). Triplicate qPCR was then performed for the enumerations of Legionella spp., L. pneumophila and MAC.

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Reviews

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Discussion

4 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

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Discussion

4 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative Legionella using Aquadien™ DNA Extraction Kit from Bio-Rad Laboratories.

Paper title

Detection of , and Mycobacterium Avium Complex (MAC) along Potable Water Distribution Pipelines

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Manufacturer protocol

Download the product protocol from Bio-Rad Laboratories for Aquadien™ DNA Extraction Kit below.

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Videos

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