Genomic DNA Purification Kit

DNA isolation / purification Bacteria - Gram negative Salmonella enterica

Experiment
DNA isolation / purification Bacteria - Gram negative Salmonella enterica
Product
Genomic DNA Purification Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
Due to low amounts of DNA present in serum samples, prolong the DNA precipitation to 1-20 hours at -20°C and reduce the volume of water for DNA dissolving to 20 µl.
Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA extraction and PCR conditions
Purification of DNA directly from water samples filtered was achieved using a Genomic DNA purification kit (Fermentas, Germany) according to the manufacturer’s instructions.

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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative Salmonella enterica using Genomic DNA Purification Kit from Thermo Fisher Scientific.

Paper title
Detection of , species, and in tap water and bottled drinking water in Isfahan, Iran
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Genomic DNA Purification Kit below.

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Videos

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