VWR Life Science RiboZol™ RNA Extraction Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- If the aqueous layer appears pink, not enough RobiZol was used	- For RT-PCR applications, DNase treatment might be required.

Protocol tips
- If the aqueous layer appears pink, not enough RobiZol was used
Downstream tips
- For RT-PCR applications, DNase treatment might be required.

Publication protocol

When applicable, RNA was isolated from myotubes grown on 6-well plates using Ribozol (Ameresco) per the manufacturer’s recommendations (http://www.amresco-inc.com/media.acux?path=/media/products/dfu/dfu-N580.pdf). Then, 500 ng to 1 μg of cDNA was synthesized using a cDNA synthesis kit (Quanta Biosciences, Gaithersburg, MD) per the manufacturer’s recommendations (http://www.quantabio.com/media/wysiwyg/pdfs/95047_qScript_cDNA_Synthesis_Kit_PPS_150319.pdf)

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Manufacturer protocol

Download the product protocol from VWR for VWR Life Science RiboZol™ RNA Extraction Reagent below.

Download manufacturer protocol

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