TriPure Isolation Reagent

RNA isolation / purification Cells - immortalized C2C12

Experiment
RNA isolation / purification Cells - immortalized C2C12
Product
TriPure Isolation Reagent from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
- For low contamination rate carefully remove the upper aqueous phase .

- Adding too little reagent can lead to contamination with DNA

Publication protocol

After adequate treatments, total RNA was extracted from C2C12 cells by Tripure Reagent (Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer's instructions. Isolated RNA was quantified using the NanoDrop ND-1000 spectrophotometer (NadroDrop Technologies, Inc., Wilmington, DE, USA) and its integrity confirmed using agarose gel electrophoresis, and stored at -80ÂșC.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized C2C12 using TriPure Isolation Reagent from Sigma-Aldrich.

Paper title
Leptin rapidly induces the expression of metabolic and myokine genes in C2C12 muscle cells to regulate nutrient partition and oxidation.
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TriPure Isolation Reagent below.

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