TriPure Isolation Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low contamination rate carefully remove the upper aqueous phase . - Adding too little reagent can lead to contamination with DNA

Protocol tips
- For low contamination rate carefully remove the upper aqueous phase . - Adding too little reagent can lead to contamination with DNA

Publication protocol

After adequate treatments, total RNA was extracted from C2C12 cells by Tripure Reagent (Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer's instructions. Isolated RNA was quantified using the NanoDrop ND-1000 spectrophotometer (NadroDrop Technologies, Inc., Wilmington, DE, USA) and its integrity confirmed using agarose gel electrophoresis, and stored at -80ºC.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TriPure Isolation Reagent below.

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