RNeasy Mini Kit

RNA isolation / purification Cells - immortalized C3H-10T1/2

Experiment
RNA isolation / purification Cells - immortalized C3H-10T1/2
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
At the appropriate timepoint(s), total RNA was harvested according to the manufacturer’s instructions (RNeasy Mini Kit; Qiagen, Valencia, CA).

Publication protocol

Cells were seeded at similar cell densities in 6-well plates as described for Alcian Blue, Alizarin Red and Oil Red O staining experiments. At the appropriate timepoint(s), total RNA was harvested according to the manufacturer’s instructions (RNeasy Mini Kit; Qiagen, Valencia, CA). Reverse transcription into cDNA was performed according to the manufacturer’s instructions (Omniscript RT Kit; Qiagen, Valencia, CA). Semi-quantitative PCR was performed for target genes using Platinum blue mastermix as per manufacturer’s instructions (Life Technologies, Carlsbad, CA).

Full paper   Login or join for free to view the full paper.

Reviews

RNeasy Mini Kit from Qiagen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized C3H-10T1/2 using RNeasy Mini Kit from Qiagen.

Paper title
Development of -Transfected C3H10T1/2 Fibroblasts for Musculoskeletal Tissue Engineering
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - immortalized C3H-10T1/2 using RNeasy Mini Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms