TRIzol Reagent

Protocol tips

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	Following incubation with 200 ng/ml BMP-2 for 1 or 4 days, total RNA was extracted using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA). RNA was also extracted from BMP-2 untreated cells. Total RNA from each sample was quantified using the NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA)and RNA integrity was assessed using standard denaturing agarose gel electrophoresis

Protocol tips

Following incubation with 200 ng/ml BMP-2 for 1 or 4 days, total RNA was extracted using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA). RNA was also extracted from BMP-2 untreated cells. Total RNA from each sample was quantified using the NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA)and RNA integrity was assessed using standard denaturing agarose gel electrophoresis

Publication protocol

Following incubation with 200 ng/ml BMP-2 for 1 or 4 days, total RNA was extracted using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA). RNA was also extracted from BMP-2 untreated cells. Total RNA from each sample was quantified using the NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA)and RNA integrity was assessed using standard denaturing agarose gel electrophoresis

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