TRIzol Reagent

RNA isolation / purification Cells - immortalized C3H-10T1/2

Experiment
RNA isolation / purification Cells - immortalized C3H-10T1/2
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Following incubation with 200 ng/ml BMP-2 for 1 or 4 days, total RNA was extracted using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA). RNA was also extracted from BMP-2 untreated cells. Total RNA from each sample was quantified using the NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA)and RNA integrity was assessed using standard denaturing agarose gel electrophoresis

Publication protocol

Following incubation with 200 ng/ml BMP-2 for 1 or 4 days, total RNA was extracted using TRIzol (Invitrogen Life Technologies, Carlsbad, CA, USA). RNA was also extracted from BMP-2 untreated cells. Total RNA from each sample was quantified using the NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA)and RNA integrity was assessed using standard denaturing agarose gel electrophoresis

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized C3H-10T1/2 using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Expression profiling of lncRNAs in C3H10T1/2 mesenchymal stem cells undergoing early osteoblast differentiation.
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Manufacturer protocol

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