TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Samples of the original R1, CGR8.8, IP14D-1 lines, derived neuronal cells (R1_Ne, CGR8.8_Ne and IP14D-1_Ne), and primary cultured neurons as positive control were harvested in TRIzol for RNA isolation. Reverse transcription of 0.5 μg total RNA produced cDNA for PCR of Pax6, Sox1, Blbp, Nestin, Tuj1, Ncam, Map2, which test whether the pluripotent stem cells differentiate into neurons.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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