TRIzol Reagent

RNA isolation / purification Cells - immortalized CGR8

Experiment
RNA isolation / purification Cells - immortalized CGR8
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Samples of the original R1, CGR8.8, IP14D-1 lines, derived neuronal cells (R1_Ne, CGR8.8_Ne and IP14D-1_Ne), and primary cultured neurons as positive control were harvested in TRIzol for RNA isolation. Reverse transcription of 0.5 μg total RNA produced cDNA for PCR of Pax6, Sox1, Blbp, Nestin, Tuj1, Ncam, Map2, which test whether the pluripotent stem cells differentiate into neurons.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized CGR8 using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Directed neuronal differentiation of mouse embryonic and induced pluripotent stem cells and their gene expression profiles.
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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