QIAamp DNA Mini Kit

DNA isolation / purification Bacteria - Gram negative Salmonella enterica

Experiment
DNA isolation / purification Bacteria - Gram negative Salmonella enterica
Product
QIAamp DNA Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

Regarding DNA extraction from bacterial
culture, the bacterial pathogens described above were
cultured in an appropriate broth medium, and DNA
was isolated from the bacterial suspensions using the
QIAamp DNA Mini Kit (Qiagen, Hilden, Germany).
The DNA samples were then used for evaluation of the
primer specificity and sensitivity of RFBS24 ver. 5 and
for comparison of the detection limits between RFBS24 and RFBS24 ver. 5. The concentrations of DNA in the
samples were determined by optical density at 260 nm
(OD260) on a NanoDrop 1000 Spectrophotometer
(Thermo Fisher Scientific, Waltham, MA, USA)

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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative Salmonella enterica using QIAamp DNA Mini Kit from Qiagen.

Paper title
An Improved Multiplex Real-Time SYBR Green PCR Assay for Analysis of 24 Target Genes from 16 Bacterial Species in Fecal DNA Samples from Patients with Foodborne Illnesses.
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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