QIAzol Lysis Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- To promote dissociation of nucleoprotein complexes, place the sample at room temperature (15–25°C) for 5 min after homogenization.		- Recommends for a cleanup of the redissolved RNA using RNeasy® Kits, which are based on silica-membrane technology, in order to remove any contaminating phenol. - If A260/A280 is low or if DNA containation is high, reduce the amount of starting material and/or increase the volume of QIAzol Lysis Reagent and the homogenization time.

Upstream tips
- To promote dissociation of nucleoprotein complexes, place the sample at room temperature (15–25°C) for 5 min after homogenization.
Downstream tips
- Recommends for a cleanup of the redissolved RNA using RNeasy® Kits, which are based on silica-membrane technology, in order to remove any contaminating phenol. - If A260/A280 is low or if DNA containation is high, reduce the amount of starting material and/or increase the volume of QIAzol Lysis Reagent and the homogenization time.

Publication protocol

Total RNA was isolated from CT26 cells using QIAzol lysis reagent (QIAGEN sciences, MD, USA) according to the manufacturer’s directions. First-strand cDNA was prepared from an RNA template (2 μg) using an oligo (dT)18 primer and a Power cDNA Synthesis Kit (iNtRon Biotech). Reverse transcription was performed at 42 °C for 50 min and then at 70 °C for 15 min. Real-time quantitative RT-PCR was performed using a Power SYBR® Green PCR Master Mix and StepOne PlusTM Real-time PCR Systems (Applied Biosystems, Foster City, CA, USA)

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Manufacturer protocol

Download the product protocol from Qiagen for QIAzol Lysis Reagent below.

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