QIAzol Lysis Reagent

RNA isolation / purification Cells - immortalized CT26

Experiment
RNA isolation / purification Cells - immortalized CT26
Product
QIAzol Lysis Reagent from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- To promote dissociation of nucleoprotein complexes, place the sample at room temperature (15–25°C) for 5 min after homogenization.
Downstream tips
- Recommends for a cleanup of the redissolved RNA using RNeasy® Kits, which are based on silica-membrane technology, in order to remove any contaminating phenol.

- If A260/A280 is low or if DNA containation is high, reduce the amount of starting material and/or increase the volume of QIAzol Lysis Reagent and the homogenization time.

Publication protocol

Total RNA was isolated from CT26 cells using QIAzol lysis reagent (QIAGEN sciences, MD, USA) according to the manufacturer’s directions. First-strand cDNA was prepared from an RNA template (2 μg) using an oligo (dT)18 primer and a Power cDNA Synthesis Kit (iNtRon Biotech). Reverse transcription was performed at 42 °C for 50 min and then at 70 °C for 15 min. Real-time quantitative RT-PCR was performed using a Power SYBR® Green PCR Master Mix and StepOne PlusTM Real-time PCR Systems (Applied Biosystems, Foster City, CA, USA)

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized CT26 using QIAzol Lysis Reagent from Qiagen.

Paper title
Arctigenin Inhibits Lung Metastasis of Colorectal Cancer by Regulating Cell Viability and Metastatic Phenotypes.
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Manufacturer protocol

Download the product protocol from Qiagen for QIAzol Lysis Reagent below.

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