TRIzol Reagent

RNA isolation / purification Cells - immortalized EL4

Experiment
RNA isolation / purification Cells - immortalized EL4
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

RNA from anti-CD3/CD28 antibody- or anti-CD3/CD28/OCILRP2 antibody-stimulated EL4 cells and normal EL4 cells were isolated using trizol reagent (15596-018, Invitrogen)

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized EL4 using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
The C-Type Lectin OCILRP2 Costimulates EL4 T Cell Activation via the DAP12-Raf-MAP Kinase Pathway
Full paper
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

Download PDF Download manufacturer protocol

Videos

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