RNeasy Mini Kit

RNA isolation / purification Cells - immortalized GL261

Experiment
RNA isolation / purification Cells - immortalized GL261
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA was extracted from the cells using the RNeasy mini kit (Qiagen, Beijing, China), followed by complimentary DNA synthesis using the Superscript III first strand synthesis kit (Invitrogen Life Technologies, Shanghai, China). qPCR was performed on Bio-Rad amplifier using the Bio-Rad real-time PCR mix (Bio-Rad, Berkeley, CA, USA). Data were analyzed using the threshold cycle value normalized to the endogenous reference gene.

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized GL261 using RNeasy Mini Kit from Qiagen.

Paper title
Study on the therapeutic effect of neural progenitor cells in mice of a glioma murine model
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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