RNeasy Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).	- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA was extracted from the cells using the RNeasy mini kit (Qiagen, Beijing, China), followed by complimentary DNA synthesis using the Superscript III first strand synthesis kit (Invitrogen Life Technologies, Shanghai, China). qPCR was performed on Bio-Rad amplifier using the Bio-Rad real-time PCR mix (Bio-Rad, Berkeley, CA, USA). Data were analyzed using the threshold cycle value normalized to the endogenous reference gene.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

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