AllPrep DNA/RNA Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Complete disruption of plasma membranes of cells and organelles is absolutely required to release all the nucleic acids contained in the sample. - Different samples require different methods to achieve complete disruption. - Incomplete disruption results in significantly reduced nucleic acid yields. - Overloading the spin columns significantly reduces nucleic acid yields.	- Homogenizing the material is necessary to redice the viscosity of the lysates caused by cell disruption. - Incomplete homogenization results in inefficient binding of DNA and RNA and therefore significantly reduced yield and purity of nucleic acids. - Excessive homogenization, on the other hand, results in shorter genomic DNA fragments. - The centrifugation temperature should be 20–25ºC. - Warm the lysate to 37ºC before transferring it to the AllPrep DNA spin column.

Upstream tips
- Complete disruption of plasma membranes of cells and organelles is absolutely required to release all the nucleic acids contained in the sample. - Different samples require different methods to achieve complete disruption. - Incomplete disruption results in significantly reduced nucleic acid yields. - Overloading the spin columns significantly reduces nucleic acid yields.
Protocol tips
- Homogenizing the material is necessary to redice the viscosity of the lysates caused by cell disruption. - Incomplete homogenization results in inefficient binding of DNA and RNA and therefore significantly reduced yield and purity of nucleic acids. - Excessive homogenization, on the other hand, results in shorter genomic DNA fragments. - The centrifugation temperature should be 20–25ºC. - Warm the lysate to 37ºC before transferring it to the AllPrep DNA spin column.

Publication protocol

Total RNA was isolated using the RNeasy Mini Kit or AllPrep DNA/RNA Mini Kit. The purity and concentration of DNA and RNA were determined by spectrophotometry.

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Manufacturer protocol

Download the product protocol from Qiagen for AllPrep DNA/RNA Mini Kit below.

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