RNeasy Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).	- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C

Publication protocol

Total RNAs from N2a cells obtained after 48 h of treatment with RSV (12.5 µM), API (12.5 µM) and RA (6.25 and 25 µM) without or with 10% FBS, were extracted and purified with the RNeasy Mini Kit (Qiagen, Courtaboeuf, France).

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

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