RNeasy Mini Kit

RNA isolation / purification Cells - immortalized NIH-3T3

Experiment
RNA isolation / purification Cells - immortalized NIH-3T3
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- If cells have low RNA content do not use more than 1 x 10*7 cells as a starting material, otherwise the RNA binding capacity of the RNeasy spin column will be exceeded.
Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C

Publication protocol

Total RNA was isolated from cells using the RNeasy mini kit (Qiagen China Co., Ltd., Shanghai, China) according to the manufacturer's instructions.

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized NIH-3T3 using RNeasy Mini Kit from Qiagen.

Paper title
TCRP1 promotes NIH/3T3 cell transformation by over-activating PDK1 and AKT1
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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