RNeasy Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- If cells have low RNA content do not use more than 1 x 10*7 cells as a starting material, otherwise the RNA binding capacity of the RNeasy spin column will be exceeded.	- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).	- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C

Upstream tips
- If cells have low RNA content do not use more than 1 x 10*7 cells as a starting material, otherwise the RNA binding capacity of the RNeasy spin column will be exceeded.
Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C

Publication protocol

Total RNA was isolated from cells using the RNeasy mini kit (Qiagen China Co., Ltd., Shanghai, China) according to the manufacturer's instructions.

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download manufacturer protocol

Videos

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