DNeasy Blood & Tissue Kit

DNA isolation / purification Tissue - brain

Experiment

DNA isolation / purification Tissue - brain

Product

DNeasy Blood & Tissue Kit from Qiagen

Manufacturer

Qiagen

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
DO NOT add bleach or acidic solutions directly to the sample-preparation waste.		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Upstream tips
DO NOT add bleach or acidic solutions directly to the sample-preparation waste.
Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

2.1. DNA extraction and PCR
DNA was extracted from the foxes’ brain tissue using the DNeasy® commercial kit for blood and tissue (Qiagen; Hilden, Germany), in accordance with the manufacturer's instructions.

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Reviews

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Discussion

5 years ago

Author: Denmark

What DNA isolation kit would work for insect samples?

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Tissue - brain using DNeasy Blood & Tissue Kit from Qiagen.

Paper title

Occurrence of Neospora caninum and Toxoplasma gondii DNA in brain tissue from hoary foxes (Pseudalopex vetulus) in Brazil.

Full paper

Manufacturer protocol

Download the product protocol from Qiagen for DNeasy Blood & Tissue Kit below.

Download manufacturer protocol

Videos

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