DNeasy Blood & Tissue Kit

DNA isolation / purification Tissue - brain

Experiment
DNA isolation / purification Tissue - brain
Product
DNeasy Blood & Tissue Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
DO NOT add bleach or acidic solutions directly to the sample-preparation waste.
Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

2.1. DNA extraction and PCR
DNA was extracted from the foxes’ brain tissue using the DNeasy® commercial kit for blood and tissue (Qiagen; Hilden, Germany), in accordance with the manufacturer's instructions.

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Reviews

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Discussion

Discussion

2 years ago

Author: Denmark

What DNA isolation kit would work for insect samples?

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Tissue - brain using DNeasy Blood & Tissue Kit from Qiagen.

Paper title
Occurrence of Neospora caninum and Toxoplasma gondii DNA in brain tissue from hoary foxes (Pseudalopex vetulus) in Brazil.
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for DNeasy Blood & Tissue Kit below.

Download PDF Download manufacturer protocol

Videos

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