NucleoSpin® RNA

RNA isolation / purification Cells - immortalized NIH-3T3

Experiment
RNA isolation / purification Cells - immortalized NIH-3T3
Product
NucleoSpin® RNA from Macherey Nagel
Manufacturer
Macherey Nagel

Protocol tips

Upstream tips
- Aliquot rDNase and store at -20 °C.
Protocol tips
- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

RNA was isolated according to the manufacturer’s protocol using Nucleo Spin RNA kit (Macherey Nagel). The isolated RNA was eluted in 40 μl RNAse free water and was examined for RNA concentration and quality by spectrophotometric measurements and gel analysis, respectively. cDNA was synthesized from isolated RNA (1 μg of transfected NIH3T3 and A549 and 0.5 μg of transfected C2C12 cells, respectively) using First Strand cDNA Synthesis Kit (Thermo Scientific) following the manufacturer’s instruction. The synthesized cDNA was stored at −20 °C.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized NIH-3T3 using NucleoSpin® RNA from Macherey Nagel.

Paper title
Human cellular CYBA UTR sequences increase mRNA translation without affecting the half-life of recombinant RNA transcripts
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Manufacturer protocol

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