TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was extracted using TRIzol Reagent® (Sigma-Aldrich; Merck KGaA). All RNA extracts were treated with DNase using a RQ1 RNase-Free DNase kit (Promega Corporation, Madison, WI, USA), according to the manufacturer's protocols.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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