TRIzol Reagent

RNA isolation / purification Cells - immortalized RIN-5F

Experiment
RNA isolation / purification Cells - immortalized RIN-5F
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

otal RNA was isolated from human insulinoma tissues stored frozen after surgery in liquid nitrogen. Briefly, about 50–100 mg of tumor tissue was homogenized in 1 mL TRIzol (Invitrogen, Lofer, Austria) and total RNA was isolated by phase-separation and precipitation with isopropanol following the manufacturers' instructions. Human pancreatic islet total RNA was obtained from the Department of Medical Biochemistry and Genetics, University of Copenhagen (Denmark) (Professor J. H. Nielsen). One μg of total RNA was reverse transcribed using SuperScript II (Invitrogen) and random primers and the resultant cDNA was diluted 1 : 3 in H2O. Of note, this part of the study had been approved by the Ethics Committee, Medical University of Vienna.

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized RIN-5F using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
The Microtubule-Associated Protein Tau and Its Relevance for Pancreatic Beta Cells
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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