TriPure Isolation Reagent

RNA isolation / purification Cells - immortalized PC-12

Experiment
RNA isolation / purification Cells - immortalized PC-12
Product
TriPure Isolation Reagent from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
- Do not wash cells before adding reagent (suspension cells).
Protocol tips
- For low contamination rate carefully remove the upper aqueous phase .

- Adding too little reagent can lead to contamination with DNA

Publication protocol

The total RNA from the PC12 cells was extracted using TriPure isolation reagent according to the manufacturer’s protocol. The RNA was then reverse transcribed using a RT-PCR kit, and real-time PCR was performed using a LightCycler Nano thermal cycler (Roche).

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized PC-12 using TriPure Isolation Reagent from Sigma-Aldrich.

Paper title
ERK5 Phosphorylates K4.2 and Inhibits Inactivation of the A-Type Current in PC12 Cells
Full paper
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TriPure Isolation Reagent below.

Download PDF Download manufacturer protocol

Videos

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