TriPure Isolation Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Do not wash cells before adding reagent (suspension cells).	- For low contamination rate carefully remove the upper aqueous phase . - Adding too little reagent can lead to contamination with DNA

Upstream tips
- Do not wash cells before adding reagent (suspension cells).
Protocol tips
- For low contamination rate carefully remove the upper aqueous phase . - Adding too little reagent can lead to contamination with DNA

Publication protocol

The total RNA from the PC12 cells was extracted using TriPure isolation reagent according to the manufacturer’s protocol. The RNA was then reverse transcribed using a RT-PCR kit, and real-time PCR was performed using a LightCycler Nano thermal cycler (Roche).

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TriPure Isolation Reagent below.

Download manufacturer protocol

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