TRIzol Reagent

RNA isolation / purification Cells - immortalized MARC-nsp11

Experiment
RNA isolation / purification Cells - immortalized MARC-nsp11
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

For PCR, cellular DNA was extracted from MARC-nsp11 cells using QIAamp DNA kit (Qiagen) and PCR was performed to determine the nsp11 gene integration. For reverse transcription (RT), total cellular RNA was extracted using Trizol (Invitrogen) and was treated with RQ1 RNase-free DNase I (Promega) followed by RT using the nsp11-specific reverse primer and PCR using the primer set

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

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Manufacturer protocol

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