RNeasy Plus Micro Kit

RNA isolation / purification Cells - immortalized HCA-7

Experiment
RNA isolation / purification Cells - immortalized HCA-7
Product
RNeasy Plus Micro Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- When processing <500 cells or <2 µg tissue, carrier RNA may be added to the lysate before homogenization.

- Foaming can be reduced by adding Reagent DX at a final concentration of 0.5% (v/v) before disruption and homogenization.

- Add 4 volumes of ethanol (96–100%) to Buffer RPE for a working solution.

Publication protocol

Total RNA was isolated using the RNeasy Plus Micro kit (catalog no. 74034; Qiagen GmbH, Hilden, Germany), according to the manufacturer's protocol.

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized HCA-7 using RNeasy Plus Micro Kit from Qiagen.

Paper title
FosB transcription factor regulates COX-2 expression in colorectal cancer cells without affecting PGE2 expression
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Micro Kit below.

Download PDF Download manufacturer protocol

Videos

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