TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Whole cell RNA preparations were carried out using TRIzol reagent (Thermo Fisher, catalog no. 15596) according to the vendor’s recommended protocol. For tumor and normal tissue, pieces were first ground finely using a mortar and pestle in liquid nitrogen prior to disruption with TRIzol reagent. Nuclear and cytoplasmic RNAs were isolated using the Cytoplasmic & Nuclear RNA Purification Kit from Norgen Biotek Corp. (catalog no. 21000) according to the vendor’s protocol. All RNA preparations were subjected to DNase treatment twice using the DNA-free kit (Thermo Fisher, catalog no. AM1906).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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