RNeasy Plus Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

otal RNA was isolated from cells using TRIzol reagent (Invitrogen) and RNeasy Plus Mini Kit (Qiagen). Genomic DNA was eliminated with RNase free DNase 1 (Roche), and total RNA was then subjected to another round of purification by phenol solution with pH4.3 ± 0.2 (Sigma-Aldrich)

For Gdf3 RNA was isolated from whole bone marrow mononuclear cells or CD45.2+ / LSK cells using TRIzol reagent (Invitrogen) and RNeasy mini kit (QIAGEN). cDNA was synthesized with Superscript First-Strand Synthesis System according to manufacturer's instructions (Invitrogen). qPCR was performed with SYBR reagent (Bio-Rad). Primers used were: Gdf3_F1 (Forward): 5’- GAA CCT GCT TCA GCT TCT CC-3’; Gdf3_R1 (Reverse): 5’-CCA TGG TCA ACT TTG CCT TT-3’. Gdf3 expression levels were normalized to that of Gapdh: Forward 5’- CCA GCC TCG TCC CGT AGA C-3’; Reverse 5’-CCC TTG ACT GTG CCG TTG-3’.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized hES(h1) using RNeasy Plus Mini Kit from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Mini Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - immortalized hES(h1) using RNeasy Plus Mini Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.