RNeasy Micro Kit

RNA isolation / purification Cells - immortalized AsPC-1

Experiment
RNA isolation / purification Cells - immortalized AsPC-1
Product
RNeasy Micro Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from up to 5x10^5 cells, or up to 5 mg of tissue.
Downstream tips
- Always blank the spectrophotometer with your elution buffer.

- When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Publication protocol

RNA was isolated from cell lysates by the RNeasy Micro Kit (Qiagen), and cDNA was synthesized from 1 ug RNA, using SuperScript III (Invitrogen) and Random Primers (Invitrogen), as per the manufacturer's protocol

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized AsPC-1 using RNeasy Micro Kit from Qiagen.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Micro Kit below.

Download PDF Download manufacturer protocol

Videos

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