TRIzol Reagent

RNA isolation / purification Cells - immortalized AsPC-1

Experiment
RNA isolation / purification Cells - immortalized AsPC-1
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

SIRT1 was examined in pancreatic cancer cells and tissues before and after GEM treatment using quantitative RT-PCR (qRT-PCR). Briefly, Total RNA (1 μg) extracted (TRIzol reagent; Invitrogen, Carlsbad, CA, USA) was reverse transcribed to cDNA according to the manufacturer's instructions. Real-time PCR was carried out using the SYBR Green quantitative PCR kit (Invitrogen) according to the manufacturer's instructions using an Applied Biosystems 7300 real-time PCR system (Carlsbad, CA, USA). SIRT1 forward primer, 5′-CGGAAACAATACCTCCACCTGA-3′; reverse primer, 5′-GAAGTCTACAGCAAGGCGAGC A-3′ (amplicon size, 242 bp). All data were normalized to the GAPDH (forward primer, 5′-GACAACTTTGGCATCGT-3′; reverse primer, 5′-ATGCAGGGATGATGTTCTGG-3′; amplicon size, 133 bp). Relative SIRT1 mRNA expression was calculated using the An external file that holds a picture, illustration, etc.
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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized AsPC-1 using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Sirtuin 1 facilitates chemoresistance of pancreatic cancer cells by regulating adaptive response to chemotherapy-induced stress
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Manufacturer protocol

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