QIAamp DNA FFPE Tissue Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA Isolation from Micro-Dissected Tissue Samples
For enrichment with tumor cells, H&E-stained slides of formalin- and HOPE-fixed samples were micro-dissected under a microscope and the tumor cells transferred to DNA isolation using Qiagen QIAmp Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instruction. In short, 5-μm-thick sections of each paraffin-block were cut on a microtome (SM 2000 R, Leica, Wetzlar, Germany), mounted on SuperFrost slides (Menzel Gläser, Braunschweig, Germany), deparaffinized by xylene and rehydrated with ethanol as following: 1 × 10 min xylene, 2 min 100% ethanol, 2 min 96% ethanol, 2 min 80% ethanol, 2 min 70% ethanol, 2 min aq.dest. The H&E-stained sections were directly used for micro-dissection without prior dehydration and addition of mounting media. Cryopreserved samples were macroscopically dissected, homogenized with liquid nitrogen in a mortar and submitted directly to DNA extraction. Purity of nucleic acids was determined using a NanoDrop 2000 spectrophotometer

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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA FFPE Tissue Kit below.

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