RNeasy Plus Mini Kit

RNA isolation / purification Cells - immortalized CAMA-1

Experiment
RNA isolation / purification Cells - immortalized CAMA-1
Product
RNeasy Plus Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA from cancer cell lines was extracted using the RNeasyPlus Mini Kit (Qiagen, Valencia, CA, USA). Total RNA measuring 1 μg was reverse transcribed in a 20 μl reaction using iScript (Bio-Rad, Hercules, CA, USA). Real time PCR for gene expression was performed with 5 μl of a 1:20 dilution of reverse transcribed cDNA using the universal probe library (UPL) system (Roche, Nutley, NJ, USA) in a LightCycler 480 (Roche). The cycling conditions were performed as per the manufacturer's instructions.

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized CAMA-1 using RNeasy Plus Mini Kit from Qiagen.

Paper title
MicroRNA-510 promotes cell and tumor growth by targeting peroxiredoxin1 in breast cancer
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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