RNeasy Micro Kit

RNA isolation / purification Cells - immortalized CAMA-1

Experiment
RNA isolation / purification Cells - immortalized CAMA-1
Product
RNeasy Micro Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from up to 5x10^5 cells, or up to 5 mg of tissue.
Downstream tips
- Always blank the spectrophotometer with your elution buffer.

- When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Publication protocol

qPCR analyses for the patients samples were performed on 150 ng total RNA isolated by RNeasy Micro Kit (Qiagen). For cell line analyses 500 ng total RNA was transcribed using First Strand cDNA Synthesis Kit (Fermentas St. Leon-Rot, Germany) together with 500 ng of universal human reference (UHR, Stratagene, Agilent technologies, Texas USA). Quantitative real-time PCR analyses were performed on Eppendorf Master Cycler using SYBR Green (Fermentas, St. Leon-Rot, Germany)

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized CAMA-1 using RNeasy Micro Kit from Qiagen.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Micro Kit below.

Download PDF Download manufacturer protocol

Videos

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