Genomic DNA isolation

DNA isolation / purification Cells - immortalized cell lines leiomyoma & myometrial cells

Experiment
DNA isolation / purification Cells - immortalized cell lines leiomyoma & myometrial cells
Product
Genomic DNA isolation from Agilent Technologies
Manufacturer
Agilent Technologies

Protocol tips

Protocol tips
Avoid removing any flocculent material when transferring the
supernatant to a 50 ml sterile tube

Publication protocol

TRF length analyses were performed as described previously (Tahara et al, 1997). Genomic DNA was purified by DNA extraction kits (Stratagene, LaJolla, CA). Three micrograms of genomic DNA was digested with the restriction endonuclease HinfI overnight at 37° C. The HinfI-digested DNA was resolved on 0.7% agarose gels and transferred to a nylon membrane (Hybond N, Amersham Corporation, Paisley, United Kingdom) under denaturing conditions. The membrane was hybridized with a 5′-32P–labeled (TTAGGG)4 telomeric oligonucleotide probe. Signal was detected by exposing the membrane to KODAK Bio Max film (Rochester, New York) with an intensifying screen.

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Discussion

Discussion

1 year ago

Author: R. Verma India

DNA isolation column clogged

During centrifugation, the column got clogged and I was unable to continue with the protocol. How can I unclog it?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Cells - immortalized cell lines leiomyoma & myometrial cells using Genomic DNA isolation from Agilent Technologies.

Paper title
Immortalization of human uterine leiomyoma and myometrial cell lines after induction of telomerase activity: molecular and phenotypic characteristics.
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Manufacturer protocol

Download the product protocol from Agilent Technologies for Genomic DNA isolation below.

Download PDF Download manufacturer protocol

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