Genomic DNA isolation

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Avoid removing any flocculent material when transferring the supernatant to a 50 ml sterile tube

Protocol tips
Avoid removing any flocculent material when transferring the supernatant to a 50 ml sterile tube

Publication protocol

TRF length analyses were performed as described previously (Tahara et al, 1997). Genomic DNA was purified by DNA extraction kits (Stratagene, LaJolla, CA). Three micrograms of genomic DNA was digested with the restriction endonuclease HinfI overnight at 37° C. The HinfI-digested DNA was resolved on 0.7% agarose gels and transferred to a nylon membrane (Hybond N, Amersham Corporation, Paisley, United Kingdom) under denaturing conditions. The membrane was hybridized with a 5′-32P–labeled (TTAGGG)4 telomeric oligonucleotide probe. Signal was detected by exposing the membrane to KODAK Bio Max film (Rochester, New York) with an intensifying screen.

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Manufacturer protocol

Download the product protocol from Agilent Technologies for Genomic DNA isolation below.

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