TRI Reagent® Sigma

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA. - The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Upstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA. - The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

Total RNA was isolated from the cells using TRI Reagent (Sigma),followed by DNase I treatment of 1 µg RNA and reverse transcription with Transcriptor High Fidelity cDNA Synthesis Kit (Roche) using lamin A/C (LMN-RT) and GAPDH-specific (GAPDH-RT) oligonucleotides.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TRI Reagent® Sigma below.

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