TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was isolated using the Trizol reagent (Invitrogen, USA) according to the manufacturer’s instructions. Total RNA was incubated with DNase I for 15 min at 37°C. One microgram of total RNA was applied into reverse transcription reaction using First Strand cDNA Synthesis Kit (Roche). One microliter of cDNA was amplified in a total reaction volume of 20 μl containing 100 nM gene specific primer and 10 μl of 2× SYBR® Green PCR Master Mix (Applied Biosystems) using StepOne Real-Time System (Applied Biosystems). The Q-PCR experiment was performed in triplicate and the mean values were used for calculations. The IGFBP-7 mRNA expression index was normalized with the reference gene GADPH using the comparative Ct method (2-△CT)

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized RPMI 2650 using TRIzol Reagent from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - immortalized RPMI 2650 using TRIzol Reagent from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.