|- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA
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1 year ago
Author: R. Verma
During centrifugation, the column got clogged and I was unable to continue with the protocol. How can I unclog it?
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